Preparation of a polyurethane membrane testosterone sensor and its application using square-wave stripping voltammetry

A novel electrochemical sensor for testosterone detection has been prepared by the chemical modification of a gold electrode (AuE). For modification, specific polyurethane (PU) films were synthesized from hexamethylene diisocyanate, olivetol, polyethylene glycol-100 (PEG100) and β-cyclodextrin. The PUs investigated as selective films, used to coat AuE surface at different concentrations thicknesses. responses modified electrodes square-wave voltammetry (SWV). One separated cathodic SWV peak was obtained –0.390 V, with PU-modified in 0.1 M phosphate buffer (PB) (pH 7.2). linearity PU over concentration range 0.1–1.0 µM (R2 = 0.995). It observed that response increased regularly sensitively increasing amount. limit, relative standard deviation sensitivity found be approximately 5.69 nM, 1.669 % 98.331 %, respectively. exhibited good selectivity low time testosterone. Therefore, offers alternative fast practical determination clinical or biomedical studies.